Actin cytoskeleton regulates ion channel activity in retinal neurons
作者:
Greg Maguire,
Victoria Connaughton,
Adriana Prat,
G Jackson,
Horacio Cantiello,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 4
页码: 665-670
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Actin cytoskeleton;Ambystoma tigrinum;Bipolar neurons;Cytochalasin D;Phalloidin;Tiger salamander;Voltage-gated K+channels
数据来源: OVID
摘要:
THE actin cytoskeleton is an important contributor to the integrity of cellular shape and responses in neurons. However, the molecular mechanisms associated with functional interactions between the actin cytoskeleton and neuronal ion channels are largely unknown. Wholecell and single channel recording techniques were thus applied to identified retinal bipolar neurons of the tiger salamander (Ambystoma tigrinu)mto assess the role of acute changes in actin-based cytoskeleton dynamics in the regulation of voltage-gated ion channels. Disruption of endogenous actin filaments after brief treatment (20–30 min) with cytochalasin D (CD) activated voltagegated K+currents in bipolar cells, which were largely prevented by intracellular perfusion with the actin filament-stabilizer agent, phalloidin. Either CD treatment under cell-attached conditions or direct addition of actin to excised, inside-out patches of bipolar cells activated and/or increased single K+channels. Thus, acute changes in actin-based cytoskeleton dynamics regulate voltagegated ion channel activity in bipolar cells.
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