Measurement of interleukin-4 and histamine in superficial cells of conjunctiva in patients with allergic conjunctivitis
作者:
FujishimaHiroshi,
SaitoIchiro,
TakeuchiTsutomu,
ShinozakiNaoshi,
TsubotaKazuo,
期刊:
Current Eye Research
(Taylor Available online 1996)
卷期:
Volume 15,
issue 2
页码: 209-213
ISSN:0271-3683
年代: 1996
DOI:10.3109/02713689608997415
出版商: Taylor&Francis
关键词: allergic conjunctivitis;conjunctival cells;histamine;interleukin-4;human
数据来源: Taylor
摘要:
Interleukin 4 (IL-4) may play a role in the development of allergic disease. We questioned whether IL-4 related phenomenon occurred on the surface of the conjunctiva during allergic conjunctivitis. Ten patients with cedar pollen-allergic conjunctivitis and 10 patients with postsurgical conjunctivitis were enrolled in this study. Cells were collected by brush cytology from the upper palpebral conjunctiva. After the cells were cultured for 24 hours, the levels of IL-4, IgE, and histamine were measured in the supernatants. An ELISA was used for histamine and a highly sensitive sandwich ELISA for IL-4 and IgE. Conjunctival cells were also stained and fixed by May-Grunwaids stain solution. The number of superficial cells, especially lymphocytes, were compared in the two groups. The total number of cells collected by brush cytology did not differ in allergic vs., postsurgical conjunctivitis. Lymphocytes were similarly present in such specimens. The levels of IL-4 (p = 0.01) and histamine (p = 0.02) significantly increased in the specimens from patients with allergic conjunctivitis, although IgE was not detected in both groups. There was a correlation between the level of IL-4 and histamine (p = 0.0001). Conjunctival cells in allergic conjunctivitis produced a larger amount of IL-4 and histamine for cedar pollen compared to the postsurgical conjunctivitis. As with other allergic diseases, IL-4 may play a role similar to that of histamine in the development of allergic conjunctivitis.
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