SEPARATION OF CLOSELY RELATED HEPTADECAPEPTIDES BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY
作者:
Huimin Yuan,
GeorgeM. Janini,
HaleemJ. Issaq,
RichardA. Thompson,
DeanK. Ellison,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 2000)
卷期:
Volume 23,
issue 1
页码: 127-143
ISSN:1082-6076
年代: 2000
DOI:10.1081/JLC-100101440
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Separations of five synthetic heptadecapeptides from the p-loop region of p21 Ras proteins, differing mostly in a single amino acid at the same position, were investigated. The studies were performed at low pHs in polyacryamide-coated capillaries, where the peptides were slightly positively charged. CZE, without surfactants, failed to separate all the peptides. Separations by MEKC employing an anionic surfactant sodium dodecyl sulfate (SDS), a neutral surfactant Tween 20 and a cationic surfactant cetyltrimethylammonium bromide (CTAB) provided results with varying degrees of success. Tween 20, slightly improved the separation while CTAB resulted in almost baseline separation of all the peptides.
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