首页   按字顺浏览 期刊浏览 卷期浏览 SEPARATION OF CLOSELY RELATED HEPTADECAPEPTIDES BY MICELLAR ELECTROKINETIC CHROMATOGRAP...
SEPARATION OF CLOSELY RELATED HEPTADECAPEPTIDES BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

 

作者: Huimin Yuan,   GeorgeM. Janini,   HaleemJ. Issaq,   RichardA. Thompson,   DeanK. Ellison,  

 

期刊: Journal of Liquid Chromatography & Related Technologies  (Taylor Available online 2000)
卷期: Volume 23, issue 1  

页码: 127-143

 

ISSN:1082-6076

 

年代: 2000

 

DOI:10.1081/JLC-100101440

 

出版商: Taylor & Francis Group

 

数据来源: Taylor

 

摘要:

Separations of five synthetic heptadecapeptides from the p-loop region of p21 Ras proteins, differing mostly in a single amino acid at the same position, were investigated. The studies were performed at low pHs in polyacryamide-coated capillaries, where the peptides were slightly positively charged. CZE, without surfactants, failed to separate all the peptides. Separations by MEKC employing an anionic surfactant sodium dodecyl sulfate (SDS), a neutral surfactant Tween 20 and a cationic surfactant cetyltrimethylammonium bromide (CTAB) provided results with varying degrees of success. Tween 20, slightly improved the separation while CTAB resulted in almost baseline separation of all the peptides.

 

点击下载:  PDF (174KB)



返 回