Autoantibodies to Poly(ADP-Ribose)Polymerase in Autoimmune Diseases
作者:
NegriC.,
ScovassiA. I.,
CerinoA.,
NegroniM.,
BorzÌR. M.,
MeliconiR.,
FacchiniA.,
MontecuccoC. M.,
Astaldi RicottiG. C.B.,
期刊:
Autoimmunity
(Taylor Available online 1990)
卷期:
Volume 6,
issue 3
页码: 203-209
ISSN:0891-6934
年代: 1990
DOI:10.3109/08916939009041040
出版商: Taylor&Francis
关键词: Connective tissue diseases;anti nuclear antibodies;poly(ADP-ribose)polymerase;ELISA;Western Blot;DNA repair and transcription
数据来源: Taylor
摘要:
Antibodies to various nuclear proteins are frequently found in sera of patients affected by connective tissue diseases and other autoimmune diseases.We investigated the specificity of circulating autoantibodies to poly(ADP-ribose)polymerase (pADPRP) in different autoimmune and connective tissue diseases: Systemic Lupus Erythematosus (SLE), Rheumatoid Arthritis (RA), Progressive Systemic Sclerosis (PSS), Sjogren's Syndrome (SS), Undifferen-tiated Connective Tissue Disease (UCTD), Cryptogenic Fibrosing Alveolitis (CFA) and Sarcoidosis.pADPRP was purified from calf thymus. Antibody specificity was detected by ELISA, western blot and enzyme activity precipitation.Positive values (mean O.D. values + 3 S.D. of 36 normal controls) were obtained in 7/15 SLE patients, 1/18 RA patients, 1/30 PSS, 3/14 SS, 0/5 UCTD, 5/21 CFA and 4/25 Sarcoidosis. The positive sera also recognized the pADPRP protein when tested by western blot. Furthermore the enzyme activity was inhibited after immunoprecipitation by some highly positive sera.
点击下载:
PDF (712KB)
返 回