Calpain in cultured bovine lens epithelial cells
作者:
LipmanRuth D.,
CyrDeanna E.,
DavidLarry L.,
TaylorAllen,
期刊:
Current Eye Research
(Taylor Available online 1991)
卷期:
Volume 10,
issue 1
页码: 11-17
ISSN:0271-3683
年代: 1991
DOI:10.3109/02713689109007606
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Calcium dependent proteolysis was examined in supernatant prepared from cultured bovine lens epithelial (BLE) cells. The presence of the calcium activated protease, calpain, was indicated by immunorecognition of 80 kDa and 30 kDa subunits of calpain in BLE cell supernatant. Degradation of125I-alpha-crystallin and FTTC labeled casein by BLE cell supernatant were shown to be calcium dependent. Inhibition of activity was achieved with EGTA, calpastatin or CbzValPheH. The data presented are the first measurement of calpain activity in cultured lens cells.
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