Improved utilization of tissue blocks for electron microscopy
作者:
P. Rømert,
M.E. Matthiessen,
期刊:
Cells Tissues Organs
(Karger Available online 1979)
卷期:
Volume 104,
issue 4
页码: 388-393
ISSN:1422-6405
年代: 1979
DOI:10.1159/000145089
出版商: S. Karger AG
关键词: Fixation;Liver cell;Ultrastructure
数据来源: Karger
摘要:
Liver tissue from miniature pig fetuses was immersion-fixed in fixative mixtures with various concentrations of formaldehyde and glutaraldehyde. The preservation quality of hepatocytes was evaluated ultrastructurally in a peripheral zone (30–130 µm below the surface) and a central zone (500 µm below the surface). In the peripheral zone the best preservation was obtained with a fixative mixture containing 2% formaldehyde and 2% glutaraldehyde and in the central zone with a fixative mixture containing 8% formaldehyde and 8% glutaraldehyde. It is concluded that a better utilization of fairly large tissue blocks for ultrastructural investigation can be obtained by division of the block and subsequent fixation in fixatives containing various concentrations of formaldehyde and glutaraldeh
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