Quantitation of Propofol Metabolite, 2,6-Diisopropyl-1,4-quinol, by Gas Chromatography-Mass Spectrometry
作者:
J. Guitton,
T. Burronfosse,
M. Sanchez,
M. Désage,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 7
页码: 1369-1378
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708007223
出版商: Taylor & Francis Group
关键词: propofol;2,6-diisopropyl-1,4-quinol;metabolism;gas chromatography-mass spectrometry
数据来源: Taylor
摘要:
Propofol is an intravenous anaesthetic widely used for induction and maintenance of anaesthesia and for sedation. A sensitive and simple gas chromatography-mass spectrometry (GC-MS) method for the determination of 2,6-diisopropyl-1,4-quinol, the major hydroxylated propofol metabolite, is presented. Thymol was used as internal standard. Thymol and 2,6-diisopropyl-1,4-quinol are extracted under basic conditions. The extraction efficiencies for quinol metabolite was > 90 %. Standard curves were linear over the ranges of 50 to 2500 ng ml−1. This assay represents an alternative to existing methods of 2,6-diisopropyl-1,4-quinol analysis, which use HPLC. The assay was applied to the analysis of propofol metabolism in vitro by rat liver microsomes.
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