Quantification of renin mRNA in various mouse tissues by a novel solution hybridization assay
作者:
Martin Paul,
Dirk Wagner,
Rainer Metzger,
Detlev Ganten,
Rudolf Lang,
Fumiaki Suzuki,
Kazuo Murakami,
J H Burbach,
Gerald Ludwig,
期刊:
Journal of Hypertension
(OVID Available online 1988)
卷期:
Volume 6,
issue 3
页码: 247-252
ISSN:0263-6352
年代: 1988
出版商: OVID
关键词: Brain renin;heart renin;gene expression;solution hybridization;pSPT18
数据来源: OVID
摘要:
A new solution hybridization assay was established for the measurement of renin mRNA. The assay makes use of a radioactively labelled renin complementary RNA as hybridization probe and allows rapid and sensitive detection of renin mRNA in amounts as low as 0.5 pg. With this assay it was possible to quantify renin mRNA in tissues with low-gene expression such as brain and heart as well as in testis, kidney and the submandibular gland (SMG) of mice. The concentrations in these organs were 0.03, 0.07, 1, 5.2 and 164 pg renin mRNA/µg RNA, respectively. These results are in agreement with Northern blotting experiments. The general applicability and ease of the solution hybridization assay described here should greatly improve the rapidity of mRNA measurements in future functional studies
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