Comparative Evaluation of an Immunofluorescent Antibody Test, Enzyme Immunoassay and Western Blot for the Detection of HIV-1 Antibody
作者:
Jeffrey P. Iltis,
Navin M. Patel,
Stephen R. Lee,
Scott L. Barmat,
William C. Wallen,
期刊:
Intervirology
(Karger Available online 1990)
卷期:
Volume 31,
issue 2-4
页码: 122-128
ISSN:0300-5526
年代: 1990
DOI:10.1159/000150146
出版商: S. Karger AG
关键词: HIV-1; Immunoassay;Immunofluorescence;HIV-1 Serology
数据来源: Karger
摘要:
Screening blood and blood products for human immunodeficiency virus type 1 (HIV-1) antibody is predominantly performed by enzyme immunoassay (EIA), and results must be confirmed by the more immunospecific Western blot (WB) assay. This study evaluated an HIV immunofluorescent antibody (IFA) test relative to WB assay for use in confirming EIA designated HIV-1 antibody-positive sera. Specimens from seroconversion and CDC panels as well as clinical specimens obtained for routine EIA HIV-1 antibody screening were evaluated. Results with 209 specimens indicated that sensitivity and specificity of the Fluorognost®-HIV assay were equivalent relative to WB. In addition, the Fluorognost-HIV IFA test was faster and easier to perform than the WB assay, and unlike the WB assay was not prone to indeterminate results
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