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Enzymatic Spectrophotometric Determination of Glycerol-3-Phosphate and Glycerophosphorylcholine.

 

作者: M. Yaqoob,   A. Nabi,   I. Hafeez,   S.Atif Ali,   M. Masoom-Yasinzai,  

 

期刊: Analytical Letters  （Taylor Available online 1997）
卷期: Volume 30, issue 1  

页码: 61-68

 

ISSN:0003-2719

 

年代: 1997

 

DOI:10.1080/00032719708002290

 

出版商: Taylor & Francis Group

 

关键词: Spectrophotometer;glycerol-3-phosphate oxidase;phospholipase-D;glycerol-3-phosphate;glycero-phosphorylcholine.

 

数据来源: Taylor

 

摘要:

A reliable enzymatic procedure has been devised for the determination of glycerol-3-phosphate and glycero-phosphorylcholine based on spectrophotometry. Glycerol-3-phosphate oxidase and phospholipase-D enzymes are used to produce hydrogen peroxide to oxidize iodide into triiodide at an elevated pH in the presence of Mo(VI), and the absorbance of the triiodide was read at 352 nm.

 

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