Improved Lipid Visualization with a Modified Osmium Tetroxide Method Using Ultrasonic Treatment and Intensification with Imidazole or Triazole
作者:
NishimuraRieko,
NakaoIezo,
ShikataNobuaki,
TsuburaAiro,
MoriiSotokichi,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1995)
卷期:
Volume 70,
issue 1
页码: 28-32
ISSN:1052-0295
年代: 1995
DOI:10.3109/10520299509108313
出版商: Taylor&Francis
关键词: lipid droplets;ultrasound;osmium tetroxide;imidazole;triazole
数据来源: Taylor
摘要:
Formalin fixed autopsy tissue containing lipids were cut into 1-5 nun thick blocks, washed well, then postfixed in 2% OsO4in 0.03 M veronal acetate buffer for 30, 60, 90, 120, or 180 min with or without ultrasonic treatment. Tissues exposed to ultrasound for 90 min showed superior penetration of OsO4and well preserved histological architecture. Tissues also were immersed for 1 hr in veronal acetate buffer (pH 7.4) containing 0.5% imidazole or triazole and compared with untreated controls. Paraffin sections, 4μm thick, were examined under a light microscope with an image analyzer. Both intensity and percentage area of osmium blackening were significantly higher in samples immersed in imidazole or triazole than in untreated controls. No difference was observed between imidazole- and triazole-immersed samples. The OsO4method, modified by ultrasound treatment and imidazole- or triazole-immersion, can be applied to routine formalin fixed autopsy materials for improved lipid visualization.
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