The occurrence of natural triploidy in two specimens ofAstyanax scabripinniscollected in the Araquá river and in the Córrego das Pedras stream, respectively, is noticed. The triploid specimen from the Araquá river presented one macro B-chromosome and the triploid specimen from Córrego das Pedras stream presented two macro B-chromosomes. The C-banding performed in the specimen from the Araquá river confirmed the triploidy and showed that the macro B-chromosome was entirely heterochromatic. Ag-NOR characterization showed two chromosomes involved in the nucleolar activity in both specimens. Some aspects related to the origin of triploid fishes are described.