Sequence of the nucleoprotein gene from a virulent British field isolate of transmissible gastroenteritis virus and its expression inSaccharomyces cerevisiae
作者:
P. Britton,
R. S. Cármenes,
K. W. Page,
D. J. Garwes,
F. Parral,
期刊:
Molecular Microbiology
(WILEY Available online 1988)
卷期:
Volume 2,
issue 1
页码: 89-99
ISSN:0950-382X
年代: 1988
DOI:10.1111/j.1365-2958.1988.tb00010.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SummarySubgenomic mRNA from a virulent isolate of porcine transmissible gastroenteritis virus (TGEV) was used to produce cDNA which was sequenced. Two non‐overlapping open reading frames (ORFs) were identified. The largest, encoding a polypeptide of 382 amino acids (relative molecular mass (Mr) 43 483), was shown to be the viral nucleoprotein gene. The second ORF, found 3’to the larger ORF, encodes a polypeptide of 78 amino acids (Mr9068) which has yet to be assigned to a viral product. The nucleoprotein gene was expressed in yeast cells under the control of two types of yeast promoters: the constitutive PGK promoter, and the inducibleGAL1promoter. Yeast cells containing recombinant plasmids, with the nucleoprotein gene in the correct orientation, produced a polypeptide of M, 47000, identical to the viral product, that reacted with a specific monoclonal antib
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