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Use of a Simple Method for the Epstein-Barr Virus Transformation of Lymphocytes from Members of Large Families of Réunion Island

 

作者: M. Ventura,   A. Gibaud,   J. Le Pendu,   D. Hillaire,   G. Gérard,   D. Vitrac,   R. Oriol,  

 

期刊: Human Heredity  （Karger Available online 1988）
卷期: Volume 38, issue 1  

页码: 36-43

 

ISSN:0001-5652

 

年代: 1988

 

DOI:10.1159/000153752

 

出版商: S. Karger AG

 

关键词: EBV;Lymphoblastoid cell line;Frozen blood;Réunion Island;H-deficient families

 

数据来源: Karger

 

摘要:

A simple method for the preparation of lymphoblastoid cell lines from small amounts (100 µl) of frozen whole blood is described. A success score greater than 90% was obtained for EBV stransformations using blood samples which had been collected several months before the infection. Due to the simplicity of the technique, up to 80 samples could be processed per day. This technique was used to prepared 242 permanent cell lines from 13 large families from Réunion Island showing blood group H deficiency. These cell lines are now available for genetic studie

 

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