Amphotericin B, 5-flucytosine (5-FC), and ketoconazole levels were estimated in vitreous and aqueous samples taken from four patients undergoing therapeutic vitrectomy for fungal endophthalmitis. The levels of amphotericin B in the vitreous of three patients were low (.04–.17µg/ml). However, 5-FC was present in a concentration of 22.2µg/ml in one patient. In another case the aqueous level of ketoconazole was 0.35µg/ml. The vitreous in the same patient contained 0.71µg/ml of the drug.