In containerless science for material processing, the acoustic fields is used to levitate and to control the position of a heated or cooled sample. The interaction between the temperature and the acoustic fields leads to complicated fluid flow phenomena, resulting in the perturbation of the sample position and the heat transfer process. The physical mechanisms in this thermal‐acoustic field were investigated using the technique of holographic interferometry and thermometry. Of particular interest was the heat transfer rate from the sample associated with the sound intensities, normal frequencies of the acoustic standing wave field, and gravitational effects. For metallic spheres with high thermal conductivity, the surface temperature was found to be uniform. The thermal flow phenomenon, which is associated with the circulating flow inside the resonant chamber, was recorded. The heat transfer coefficient at the sample surface was correlated with the acoustic and the gravitational parameters, based on the classical theory of convective heat transfer. These correlations can be used to predict the heat transfer from a spherical object in a zero‐gravity environment.