Simultaneous Determination of Timolol Maleate and Pilocarpine Hydrochloride in Ophthalmic Solutions by First Derivative UV Spectrophotometry and PLS-1 Multivariate Calibration
作者:
Maria L. Satuf,
Juan C. Robles,
Héctor C. Goicoechea,
Alejandro C. Olivieri,
期刊:
Analytical Letters
(Taylor Available online 1999)
卷期:
Volume 32,
issue 10
页码: 2019-2033
ISSN:0003-2719
年代: 1999
DOI:10.1080/00032719908542949
出版商: Taylor & Francis Group
关键词: Absorption spectrophotometry;First-derivative/zero-crossing;Multivariate calibration;Partial least-squares;Timolol maleate;Pilocarpine hydrochloride
数据来源: Taylor
摘要:
The use of UV spectrophotometry (first-derivative/zero-crossing and zero-order spectra/multivariate calibration) is reported for the analysis of two miotic agents in ophthalmic solutions. The resolution of these mixtures has been accomplished without prior separation or derivatisation by using: 1) first-derivative measurements at two appropriate zero-crossing points: λ1= 222 nm, where the absorption corresponding to excipients is negligible, and λ2= 307 nm, where the contribution of pilocarpine and excipients to the overall absorption is negligible, and 2) partial least squares (PLS-1) regression analysis of zero-order spectral data. Although the components show an important degree of spectral overlap, they have been simultaneously determined with high accuracy, and with no interference from ophthalmic solution excipients.
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