Germination ofPterocarpus angolensisDC. and evaluation of the possible antimicrobial action of the phloem sap
作者:
K. van der Riet,
L. van Rensburg,
R.I. De Sousa Correia,
L.J. Mienie,
G.H.J. Krüger,
期刊:
South African Journal of Plant and Soil
(Taylor Available online 1998)
卷期:
Volume 15,
issue 4
页码: 141-146
ISSN:0257-1862
年代: 1998
DOI:10.1080/02571862.1998.10635132
出版商: Taylor & Francis Group
关键词: Antimicrobial activity;dry heat;gibberellin;Pterocarpus angolensis;seed germination
数据来源: Taylor
摘要:
The Transvaal teak (Pterocarpus angolensis)is being over utilised in its natural habitat, although it is a protected tree in South Africa, Swaziland and Namibia. The phloem sap of this species has several traditional, medicinal uses and the wood is used primarily for furniture and fuelwood. The aim of this study was firstly, to determine which seed pre-treatments would ensure optimal germination and secondly, to evaluate the potential antimicrobial activity of the phloem sap. The initial germination trial was conducted by using unsterilised seeds in the dark, which resulted in low germination rates (7.78%) and high percentages of fungal infections (45.56%), primarilyAspergillus nigerand aFusariumsp. In the second experiment the seeds were sterilised with a mixture of 60 g Thiram and 70 g Captan and germinated in an alternating light/dark environment. In both experiments the seeds were subjected to either a hormonal, heat or control pre-treatment and were allowed to germinate at three different temperature regimes. Different sap concentrations were tested for antimicrobial activity against both gram positive (Staphylococcus aureus) and gram negative (Escherichia coli; Pseudomonas aeruginosa)procaryotes as well as two eucaryotes (Candida albicans; Aspergillus niger), with negative results. The phloem sap was analysed by means of GC-MS and a few compounds, which have been identified, are reported.
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