Identification of the Plant Nucleolonema Using Silver Staining of Serial Ultrathin Sections
作者:
SatoSeiichi,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 3
页码: 149-152
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110025
出版商: Taylor&Francis
关键词: nucleolonema;silver staining;ultrathin section;Vicia faba
数据来源: Taylor
摘要:
A simple silver staining technique was applied to serial ultrathin sections ofVicia fabaroot tip meristems to investigate the structural organization of the nucleoli. The procedure consists of three steps: hydrophilic Lowicryl resin embedding, preparation of ribbons of serial ultrathin sections, and silver impregnation. This technique provided good contrast for a filamentous structure, previously called the“nucleolonema”, in nucleoli at the light microscope level. Silver appears to react with some acidic proteins involved with ribosomal RNA transcription, since the major constituents of the nucleolonema are believed to be active rRNA transcriptional units. Tracing the nucleolonema through serial ultrathin sections strongly suggests that the nucleolonema is meandering and partly coiling within the nucleolus. The present technique permits identification of the nucleolonema in other plant species and investigation of its three-dimensional structural organization.
点击下载:
PDF (640KB)
返 回