Time-Resolved Fluorescence Receptor Assay for Benzodiazepines
作者:
Toshifumi Takeuchi,
Midori Yoshida,
Yozo Kabasawa,
Ritsuko Matsukawa,
Eiichi Tamiya,
Isao Karube,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 7
页码: 1535-1545
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017430
出版商: Taylor & Francis Group
关键词: Benzodiazepine receptor;Time-resolved fluorometry;Non-isotopic receptor assay;Europium chelate;1012-S
数据来源: Taylor
摘要:
A novel non-isotopic receptor binding technique for the detection of benzodiazepines is described. A benzodiazepine labeled with europium chelate was prepared and employed as a labeled ligand, and time-resolved measurements of the long lifetime fluorescence of europium chelate allowed avoidance of interference due to proteins in the receptor preparation. Experimental results demonstrate a sigmoid inhibition curve, with binding of the labeled ligand inhibited by comparable concentrations of the unlabeled drug. The proposed assay may provide a simple procedure for the measurement of benzodiazepines in biological systems and a useful tool in the screening of natural substances for new classes of benzodiazepine-like compounds.
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