Rapid Method for the Determination of Diaminopimelic Acid Using Ion Exchange Column Chromatography
作者:
Janos Csapó,
Zsuzsanna Csapo-Kiss,
Endre Csordás,
TrumanG. Martin,
Staffan Folestad,
Anna Tivesten,
Sandor Némethy,
期刊:
Analytical Letters
(Taylor Available online 1995)
卷期:
Volume 28,
issue 11
页码: 2049-2061
ISSN:0003-2719
年代: 1995
DOI:10.1080/00032719508000024
出版商: Taylor & Francis Group
关键词: diaminopimelic acid;DAPA;fast method;oxidation with performic acid;bacterial protein
数据来源: Taylor
摘要:
A new method for determinating diaminopimelic acid (DAPA) from rumen fluid was developed. The concentration of DAPA is used as an indicator for the estimation of protein content of bacterial origin. Due to the performic acid oxidation preceding hydrolysis of proteins, the neighbouring amino acids do not interfere in the determination of DAPA. As a result, even trace concentrations of DAPA may be accurately determined. Since, following the performic acid oxidation the sample does not contain methionine, the buffers developed for rapid determination of methionine may be used to advantage. As a result of this, DAPA may be determined by ion exchange coílumn chromatography in ca. 18 minutes. Following the development of the analytical method, it was applied to the determination of DAPA in the rumen fluid and the bacterial proteins prepared from the rumen fluid of cattle, goats and sheep. Based on the results, a method for evaluation of protein content of bacterial origin, based on the DAPA content, was derived.
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