Sodium–hydrogen antiporter protein in normotensive Wistar–Kyoto rats and spontaneously hypertensive rats
作者:
Martin Siczkowski,
Joan Davies,
Leong Ng,
期刊:
Journal of Hypertension
(OVID Available online 1994)
卷期:
Volume 12,
issue 7
页码: 775-782
ISSN:0263-6352
年代: 1994
出版商: OVID
关键词: Sodium-hydrogen antiport;hypertension;vascular smooth muscle cell.
数据来源: OVID
摘要:
ObjectiveTo examine the mechanism of increased Na–H antiport activity in tissues of the spontaneously hypertensive rat (SHR) by measuring the amount of sodium–hydrogen exchanger isoform 1 (NHE-1) in cultured vascular and striated muscle cells, and in exvivotissue extracts of membranes from the brain, heart, kidney and skeletal muscle.MethodsA polyclonal rabbit antibody was raised against a fusion protein consisting of a section of the carboxyl tail of NHE-1 and β-galactosidase. Cell extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, and proteins were transferred to supported nitrocellulose. NHE-1 was detected by Western blotting and quantified by densitometry.ResultsCultured aortic and striated muscle cells from SHR contained similar amounts of NHE-1 on Western blots to those from control Wistar-Kyoto (WKY) rat cells.Ex vivoextracts of crude membranes from SHR tissues also contained quantities of NHE-1 similar to those from WKY rat tissues.ConclusionThe increased Na–H antiport activity observed in SHR cellsin vitroandin vivois not due to an increased amount of NHE-1 protein in SHR cells. This suggests that in this model of hypertension the increased transport activity results from an increased turnover number per NHE-1 molecule.
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