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Reaction of Amino Acid Esters and Amides witho-Phthalaldehyde. Limitations in Fluorescent Yield

 

作者: PierreDe Montigny,   LarryA. Sternson,  

 

期刊: Analytical Letters  （Taylor Available online 1984）
卷期: Volume 17, issue 16  

页码: 1893-1902

 

ISSN:0003-2719

 

年代: 1984

 

DOI:10.1080/00032718408077191

 

出版商: Taylor & Francis Group

 

数据来源: Taylor

 

摘要:

Amino acid esters and amides fail to produce strong fluorescence following their reaction witho-phthalaldehyde (OPA) in the presence of β-mercaptoethanol. The lack of luminescence appears to arise from a quenching phenomenon rather than from reduced reactivity of these substrates toward OPA. The amido hydrogen in the peptide linkage has been implicated in the loss in fluorescence yield.

 

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