Plant tissue analysis can be utilized by golf course superintendents to monitor a fertility program and to determine the nutrient status of turfgrass. The objectives of this study were to compare washed and unwashed plant tissue samples and to assess the nutrient status of golf course putting greens of creeping bentgrass (Agrostis palustrisHuds. ‘Penncross'). Turfgrass clippings were collected monthly from 18 putting greens from January through June, 1993. A portion of each sample was washed with deionized water, while the remainder of the sample was not washed. Plant tissue was dried at 70°C for 72 hr at which time dry weights were determined. Samples of both washed and unwashed plant tissue were analyzed for both macro‐ and micronutrient composition. There were differences between the washed and unwashed tissue samples for macro‐ and micronutrients for certain months. The range in concentration for the unwashed samples for a given nutrient in a given month was generally greater than the range in values for the washed sample of the given nutrient. This indicates the possibility of soil contamination from cultural practices and/or course player use for the unwashed samples. Nutrient analyses of the washed plant tissue samples gave a more consistent and reliable status of the nutrient content of creeping bentgrass on the greens.