Detectionin vivo6 hours after immunization of a mediator with possible antisuppressor activity
作者:
Azzam Al‐Maghazachi,
Sho‐Tone Lee,
Frixos Paraskevas,
期刊:
European Journal of Immunology
(WILEY Available online 1983)
卷期:
Volume 13,
issue 10
页码: 799-804
ISSN:0014-2980
年代: 1983
DOI:10.1002/eji.1830131004
出版商: WILEY‐VCH Verlag GmbH
数据来源: WILEY
摘要:
AbstractImmunization of mice with foreign proteins or particulate antigen induces the formation of immunoglobulin (Ig)‐antigen complexes which are strongly cytophilic for T cells and have been shown recently to markedly enhance the 7 S antibody response. In this report we demonstrate that pretreatment of the animals with cyclophosphamide or anti‐I‐J antiserum eliminates the difference in the antibody response between the mice injected with the complexes and the controls, in other words, the enhancement. Six hours after allogeneic stimulation the serum of mice contains also a cytophilic (for T cells) Ig which most likely represents, as in the case of the foreign antigens, complexes of Ig with alloantigens. The allogeneically induced 6‐h serum (6HS) contains a factor which enhances the cytotoxic T lymphocyte(s) (CTL) responsein vivo.As with the 7 S antibody response, pretreatment of mice with cyclophosphamide, in doses known to eliminate suppressor cell expression, “masks” the CTL enhancement of the allogeneically induced 6HS. The same result was also observed with the anti‐I‐J antiserum.In conclusion, the 6‐h complexes in cyclophosphamide‐ and anti‐I‐J‐treated mice do not produce a 7 S antibody or CTL response above that produced by the control group (no complexes).Using heat‐treated allogeneic tumor cells, known to induce suppressor cellsin vivo, we have shown that for a low dose of tumor cells, the allogeneically induced 6HS did not block the induction of suppressor cells, but completely blocked their function. These results suggest that the enhancement of both humoral and cell‐mediated immunity by the 6HS (complexes) is likely to be due to interference with normal suppressor cell function, that is
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