Cerebrospinal Fluid of Newborn Infants Contains a Deglycosylated Form of the Intermediate Filament Nestin
作者:
GRIGELIONIENÉ GIEDRÉ,
BLENNOW MATS,
TÖRÖK CARINA,
FRIED GABRIEL,
DAHLIN INGRID,
LENDAHL URBAN,
LAGERCRANTZ HUGO,
期刊:
Pediatric Research
(OVID Available online 1996)
卷期:
Volume 40,
issue 6
页码: 809-814
ISSN:0031-3998
年代: 1996
出版商: OVID
数据来源: OVID
摘要:
Nestin is an intermediate filament protein found in CNS progenitor cells. Nestin reappears in CNS tumor cells and reactive astrocytes after CNS injury. In this study we investigated whether nestin could be detected in the cerebrospinal fluid (CSF) of newborn infants and whether expression levels change with gestational age (GA) and/or brain injury. Using Western blot analysis, we examined the expression of nestin in the CSF of newborn infants(GA 25-42 wk) with asphyxia (n= 14), periventricular leukomalacia and peri(intra)ventricular hemorrhage (n= 7), and in a control group (n= 11). Protein extract from the periventricular brain tissue of a 1-wk-old infant was also analyzed. Nestin was detected in all the CSF samples and in the protein extract from the periventricular brain tissue. Although the CSF levels of nestin expression did not change with increasing GA, the asphyxia group had significantly lower levels of nestin in the CSF. An unexpected finding was that brain-derived nestin had an apparent molecular mass of approximately 240 kD, whereas all analyzed CSF samples contained two nestin-immunoreactive proteins at 200 and 220 kD. Experimental deglycosylation of the 240-kD form reduced the molecular mass to 220 kD, indicating that nestin undergoes a specific deglycosylation upon release into the CSF.Abbreviations: CSF,cerebrospinal fluid;IF,intermediate filament;PVL,periventricular leukomalacia;PIVH,peri(intra)ventricular hemorrhage;GA,gestational age;PCR,polymerase chain reaction
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