AbstractThe effect of voltage on the rate of desorption of radiolabeled rabbit immunoglobulin G (IgG) from protein A‐Sepharose was studied. In the absence of voltage, the rate of desorption was measured using a vast excess of unlabeled IgG to prevent re‐adsorption. Electrophoretic desorption was measured using a column equipped with electrodes and with eluent flow occurring in the direction of electrophoresis. The rate of desorption was very sensitive to voltage, and, furthermore, desorption could occur at a rate 25 times faster than in the absence of a voltage. The results are interpreted to indicate that the presence of voltage not only altered the equilibrium of the system but altered the equilibrium or rate constants of the sys