Assay of Serum Glutamic-Oxaloacetic Transaminase Using Malate Dehydrogenase Preparation Obtained fromStreptomyces aureofaciens
作者:
H. Yamanaka,
C. Laluce,
G. de Oliveira-Neto,
期刊:
Analytical Letters
(Taylor Available online 1995)
卷期:
Volume 28,
issue 13
页码: 2305-2316
ISSN:0003-2719
年代: 1995
DOI:10.1080/00032719508000374
出版商: Taylor & Francis Group
关键词: Transaminase;SGOT;malate dehydrogenase;Streptomyces
数据来源: Taylor
摘要:
A modified spectrophotometric method for serum glutamic-oxaloacetic transaminase (SGOT) assay was developed. A crude cell-free extract fromStreptomyces aureofacienswhich showed a high level of malate dehydrogenase (MDH) activity (E.C. 1.1.1.37) was used as the enzymatic indicator. The lyophilized microbial preparation was used without previous purification and was quite stable under refrigeration for one year. Serum sample assays using both the method utilizing the crude cell extract and an enzymatic commercial kit showed good correlation.
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