A method is described for the determination of ferrocene and its derivatives by the fluorescence quenching of europium-2-benzoyl-indan-1,3-dione-cetyltrimethyl-ammonium bromide(CTMAB) system. Under the optimum conditions, the decrease of the fluorescence intensity was a linear function of the concentration of ferrocene or its derivatives in the range 1.0 × 10−6-1.0 × 10−5mol/L. The detection limit was 1.0 × 10−7mol/L. The system was used for the determination of ferrocene n-heptyl β-diketone, with satisfactory results.