In Situ Hybridization Analysis for Herpes Simplex Virus Nucleic Acids in Alzheimer Disease
作者:
Douglas Walker,
John O'Kusky,
Patrick McGeer,
期刊:
Alzheimer Disease and Associated Disorders
(OVID Available online 1989)
卷期:
Volume 3,
issue 3
页码: 123-131
ISSN:0893-0341
年代: 1989
出版商: OVID
关键词: Herpes simplex virus;In situ hybridization;Nucleic acids
数据来源: OVID
摘要:
Histological sections of brain from patients showing evidence of advanced pathology of Alzheimer disease (AD) were examined for the presence of herpes simplex type-1 (HSV-1) nucleic acids by a sensitive in-situ hybridization technique. Samples from neurologically normal patients were examined in parallel. Sensitivity of the assay was verified by the detection of HSV-1 nucleic acids in neurons of trigeminal ganglia taken from cases of AD and normal controls. This indicated that the hybridization reaction was sufficiently sensitive to detect latent HSV-1 infections. Positive hybridization in the brain was only detected in a confirmed case of herpes simplex virus encephalitis. These results appear to confirm previous reports that HSV-1 infection is not directly involved in the pathology associated with AD.
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