Determination of Phosphatidylcholine Hydroperoxide in Human Plasma by Chemiluminescence - High Performance Liquid Chromatography
作者:
Teruo Miyazawa,
Keiichi Yasuda,
Kenshiro Fujimoto,
Takashi Kaneda,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 6
页码: 1033-1044
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808071929
出版商: Taylor & Francis Group
关键词: Chemiluminescence;HPLC;human plasma;phosphatidylcholine hydroperoxide
数据来源: Taylor
摘要:
To determine phosphatidylcholine hydroperoxide (PCOOH) in human plasma lipids, a chemiluminescence -high performance liquid chromatography (CL-HPLC) system was newly developed and applied. This system * To whom correspondence should be addressed. consisted of separation of phosphatidylcholine derivatives with normal phase HPLC and detection of hydroperoxide-specific chemiluminescence of PCOOH. By use of this system, the PCOOH in normal human plasma was quantitatively assayed to be 28-431 pmol hydroperoxide-02 per ml of plasma. The high sensitivities, reproducibilities and specificities of the CL-HPLC assay were shown for determining plasma PCOOH.
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