Cryofracturing as a Technique for the Study of Fungal Structures in the Scanning Electron Microscope
作者:
O'DonnellK. L.,
HooperG. R.,
期刊:
Mycologia
(Taylor Available online 1977)
卷期:
Volume 69,
issue 2
页码: 309-320
ISSN:0027-5514
年代: 1977
DOI:10.1080/00275514.1977.12020062
出版商: Taylor&Francis
数据来源: Taylor
摘要:
SUMMARYWhen freeze-fractured fungal structures are critical point dried and then examined in the scanning electron microscope a number of structures are evident that are obscured by other techniques. A comparison of freeze-fracture to the techniques of razor blade sectioning and microtomy of wax-embedded or plastic-embedded material was carried out or reviewed from the literature. In most cases superior results can be obtained with the cryofracture technique followed by critical point drying.
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