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Cryofracturing as a Technique for the Study of Fungal Structures in the Scanning Electron Microscope

 

作者: O'DonnellK. L.,   HooperG. R.,  

 

期刊: Mycologia  (Taylor Available online 1977)
卷期: Volume 69, issue 2  

页码: 309-320

 

ISSN:0027-5514

 

年代: 1977

 

DOI:10.1080/00275514.1977.12020062

 

出版商: Taylor&Francis

 

数据来源: Taylor

 

摘要:

SUMMARYWhen freeze-fractured fungal structures are critical point dried and then examined in the scanning electron microscope a number of structures are evident that are obscured by other techniques. A comparison of freeze-fracture to the techniques of razor blade sectioning and microtomy of wax-embedded or plastic-embedded material was carried out or reviewed from the literature. In most cases superior results can be obtained with the cryofracture technique followed by critical point drying.

 

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