In this study, we reported a chromatographic protocol using C18silica gel phase and basic Al2O3phase to research the creating and recovery of taxol from the extract ofTaxus cuspidatecallus cultures. With the cleaning up by basic Al2O3chromatography, the content of taxol determined in our experiment was increased greatly. The main source of increasing taxol concentration was the isomerization of 7-epi-taxol to taxol; taxol could also be decomposed to baccatin III and 10-deacetylbaccatin III in the Al2O3column. At the optimized conditions, the total recovery of taxol from the extract was more than 160%.