Induction of Synchronized Fissions in Telotrochidium henneguyi
作者:
DAVID McLAUGHLIN,
DONALD T. BARNES,
HAROLD E. FINLEY,
期刊:
The Journal of Protozoology
(WILEY Available online 1970)
卷期:
Volume 17,
issue 1
页码: 12-15
ISSN:0022-3921
年代: 1970
DOI:10.1111/j.1550-7408.1970.tb05153.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
SYNOPSIS.Cultures ofTelotrochidium henneguyi, begun with logarithmic phase cells, were employed in an effort to produce synchronized fission by heat treatment. The cells tolerated a temperature range of 20–50 C; temperatures above 50 were lethal. When cells were exposed to a single shock for 30 min, 30–40 produced 0–50% encystment with total excystment after 10 min exposure to room temperature (heat shock range). No encystment occurred between 20–30 (intershock range). Encystment and excystment time varied directly with temperature between 40–50.The most effective procedure for inducing synchronized fission consisted of 6 cycle program of 38/28 C (shock temperature/intershock temperature) administered for 15/15 (shock/intershock duration in min). Division indices (DI = cells dividing/total population X 100 =%) ranged from 12–66% with a mean of 37.25%. In control cells, division indices ranged from 2–20% with an average of 12%. Inferences from these independently derived findings
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