Inhibition of the glycolytic pathway by methylglyoxal in human platelets
作者:
G. Leoncini,
M. Maresca,
E. Buzzi,
期刊:
Cell Biochemistry and Function
(WILEY Available online 1989)
卷期:
Volume 7,
issue 1
页码: 65-70
ISSN:0263-6484
年代: 1989
DOI:10.1002/cbf.290070111
出版商: John Wiley&Sons, Ltd.
关键词: Platelets;glucose metabolism;glycolysis;methylglyoxal
数据来源: WILEY
摘要:
AbstractThe incubation of human platelets with methylglyoxal and glucose produces a rapid transformation of the ketoaldehyde toD‐lactate by the glyoxalase system and a partial reduction in GSH. Glucose utilization is affected at the level of the glycolytic pathway. No effect of the ketoaldehyde on glycogenolysis and glucose oxidation through the hexose monophosphate shunt was demonstrated. Phosphofructokinase, fructose 1,6 diphosphate (F1, 6DP) aldolase, glyceraldehyde 3‐phosphate dehydrogenase and 3‐phosphoglycerate mutase were mostly inhibited by methylglyoxal. A decrease in lactate and pyruvate formation and an accumulation of some glycolytic intermediates (fructose 1,6 diphosphate, dihydroxyacetone phosphate, 3‐phosphoglycerate) was observed. Moreover methylglyoxal induced a fall in the metabolic ATP concentration. Since methylglyoxal is an intermediate of the glycolytic bypass system from dihydroxyacetone phosphate toD‐lactate, it may be assumed that ketoaldehyde exerts a regulating effect on triose m
点击下载:
PDF
(435KB)
返 回