Progress in application of molecular biological and genetic engineering techniques as a new successful approaches for genetic improvement of the existing grapevine varieties and rootstocks are discussed. The regenerative procedure efficiency and the development of a successful transformation system are species dependent. Agrobacterium mediated gene transfer approach is more useful since now to grape. Beneficai genes encoding for proteins that are involved in disease's resistance have been inserted into established grape cultivars and rootstocks. Transgenic plants expressing selectable genes were obtained and integration of foreign genes was verified by hystochemical GUS assay, RCR assay and Southern blot analysis. The propagation of genetically manipulated grape plants is in the way and their places in breeding programs are also considered.