Single DNA molecule grafting and manipulation using a combined atomic force microscope and an optical tweezer
作者:
G. V. Shivashankar,
A. Libchaber,
期刊:
Applied Physics Letters
(AIP Available online 1997)
卷期:
Volume 71,
issue 25
页码: 3727-3729
ISSN:0003-6951
年代: 1997
DOI:10.1063/1.120495
出版商: AIP
数据来源: AIP
摘要:
In this letter, we report on spatially selecting and grafting a DNA-tethered bead to an atomic force microscope (AFM) cantilever, using an optical tweezer. To quantify this technique, we measure force versus extension of a single DNA molecule using AFM. For such studies, we have developed a micromanipulation approach by combining an AFM, an optical tweezer, and visualization setup. The ability to select a single DNA polymer and specifically graft it to a localized position on a substrate opens up new possibilities in biosensors and bioelectronic devices. ©1997 American Institute of Physics.
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