Partial Characterization of Endogenous Digoxinlike Substance in Human Urine
作者:
Ellen Vinge,
Rolf Ekman,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 1988)
卷期:
Volume 10,
issue 1
页码: 8-15
ISSN:0163-4356
年代: 1988
出版商: OVID
关键词: Digoxin;Digoxinlike substance;Radioimmunoassay;Bioassay;Human urine.
数据来源: OVID
摘要:
Urinary samples were collected from individuals not taking cardiac glycosides. Aliquots of 30 ml were passed through preparative octadecylsilane-bonded phase columns and eluted in fractions by stepwise increasing concentrations of acetonitrile. Eluted fractions were analysed for their contents of endogenous digoxinlike substance (EDLS) by radioimmunoassay of digoxin and by a bioassay of cardiac glycosides, which measures the uptake of rubidium (86Rb) by erythrocytes as an index of Na+, K+-ATPase activity. In both assays, digoxinlike activity was found in several fractions, but the highest values were consistently measured in the fractions eluted with 40% acetonitrile. Greater amounts of EDLS were recovered from the urine of pregnant women than from the urine of men and nonpregnant women.
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