Adherence of Rfd-1 Positive Dendritic Cells to the Basal Surface of Thyroid Follicular Cells in Graves' Disease
作者:
MolneJohan,
JanssonSvante,
EricsonLarse,
NilssonMikael,
期刊:
Autoimmunity
(Taylor Available online 1994)
卷期:
Volume 17,
issue 1
页码: 59-71
ISSN:0891-6934
年代: 1994
DOI:10.3109/08916939409014659
出版商: Taylor&Francis
关键词: Thyroid gland;dendritic cells;HLA-DR;RFD-1;Graves' disease;autoimmunity;cell adhesion
数据来源: Taylor
摘要:
HLA-DR positive cells infiltrating Graves' thyroid tissue were examined for their expression of cell-specific immunological markers using light and electron microscopic immunostaining of frozen sections and isolated open thyroid follicles. Graves' glands (n = 21) were enriched of CD68 and Leu-M5/CD1 lc positive monocytes/macrophages as well as CD4 and CD8 lymphocytes. These cell types were distributed juxtafollicular as well as in other tissue areas. Only the RFD-1 antibody, considered to label antigen-presenting cells including dendritic cells, identified cells invariably located close to the interstitial follicular surface. After follicle isolation, RFD-1 cells were enriched compared to Leu-M5 cells and exclusively adherent to the follicular epithelium. The plasma membrane of RFD-1 positive cells were in intimate contact with the basolateral membrane of the thyrocytes, sometimes extending deeply into the intercellular space of the epithelium. Parallel labelling experiments suggested that the follicle-adhering RFD-1 cells also expressed HLA-DR. Our findings show that in human thyroid glands with Graves' disease RFD-1 positive cells with a dendritic morphology establish direct contact with the follicular epithelium. In view of the fact that both HLA-DR and RFD-1 are associated with antigen-presenting functions it is suggested that a direct interaction of dendritic cells with thyrocytes might be an important component of the autoimmune reaction in Graves' disease.
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