Immunohistochemical Demonstration of proGnRH and GnRH in the Preoptic-Basal Hypothalamus of the Primate
作者:
Oline K. Ronnekleiv,
John P. Adelman,
Eckard Weber,
Edward Herbert,
Martin J. Kelly,
期刊:
Neuroendocrinology
(Karger Available online 1987)
卷期:
Volume 45,
issue 6
页码: 518-521
ISSN:0028-3835
年代: 1987
DOI:10.1159/000124785
出版商: S. Karger AG
关键词: ProGnRH;Cell soma;Preoptic-basal hypothalamus;Macaque;Baboon
数据来源: Karger
摘要:
An antiserum (ARK-1) specific to the gonadotropin-releasing hormone precursor (proGnRH) was produced by immunizing with a synthetic peptide (proGnRH 6–16; Gly-Leu-Arg-Pro-Gly-Gly-Lys-Arg-Asp-Ala-Glu) which bridges the proteolytic cleavage site of proGnRH. When used in the radioimmunoassay, ARK-1 bound 25% of the iodinated 5–16 fragment at a 1:30,000 dilution with a sensitivity of 1 pg/tube. Using immunohistochemical techniques, we observed that in serial and the same sections through the preoptic-basal hypothalamus (POA-BH), the precursor molecule was primarily present in the cell soma, whereas GnRH was found in the cell soma, nerve fibers, and terminals of the same neurons. These data indicate that the processing of proGnRH to biologically active peptides (e.g., GnRH) in the rhesus macaque and the baboon POA-BH primarily occurs in the cell s
点击下载:
PDF
(762KB)
返 回