Potassium Channel Openers Stimulate DNA Synthesis in Mouse Epidermal Keratinocyte and Whole Hair Follicle Cultures
作者:
Charles S. Harmon,
Diane Lutz,
Janet Ducote,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1993)
卷期:
Volume 6,
issue 3
页码: 170-178
ISSN:1660-5527
年代: 1993
DOI:10.1159/000211131
出版商: S. Karger AG
关键词: Keratinocytes;Hair follicles;Hair growth;Potassium channel openers
数据来源: Karger
摘要:
We have conducted studies using primary mouse epidermal keratinocyte and whole hair follicle cultures to investigate the mechanism of the hypertrichotic activity of potassium channel openers. In a time course study, the extent of stimulation of epidermal keratinocyte DNA synthesis by minoxidil increased as the rate of DNA synthesis in control cultures declined. Minoxidil stimulation of DNA synthesis in 7-day cultures required prolonged (> 1 day) exposure to the agent. Pinacidil and diazoxide also stimulated DNA synthesis in mouse epidermal keratinocyte cultures. In addition, minoxidil, pinacidil, diazoxide, and cromakalim stimulated DNA synthesis in whole-organ cultures of mouse hair follicles. These results suggest that potassium channel openers retard the loss of proliferative activity of differentiating keratinocytes and support the hypothesis that these agents stimulate hair growth through a direct effect on hair follicles.
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