Humus formations from forest and brushwood ecosystems on calcareous substrates in representative sites from Sierra de Gador in Southern Spain were analyzed. The humus composition, as determinated by three independent fractionation procedures, suggests that soil organic matter turnover is not related to the vegetation biotypes, but to a series of local factors among which climatic and soil characteristics predominate. Of the parameters studied, the amount of fulvic acids, and to a lesser extent, the relative proportion of soil perylenequinonic pigments (P‐type humic acids), reflected differences in altitude. The percentage of nitrogen (N) in humic acids was the parameter that was most informative of the characteristics of humus. This finding contrasts with the poorly differentiated patterns of the particulate organic matter fractions, whose evolution is probably independent of environmental factors because of physical protection by soil carbonates. The carbon dioxide (CO2) release curves reproduced under laboratory conditions showed that the intrinsic biodegradability of soil organic matter does not reflect the nature of the plant residues in the sites sampled (170–2240 m), but is weakly dependent on climatic factors. We postulate that the calcium saturation, together with the active participation of inorganic colloids, force the convergent evolution of soil humus by compensating most of the effects of the climatic and biotic factors, and the historic influence of human activity in the area studied.