Partial lithium‐associated protection against apoptosis induced by C2‐ceramide in cerebellar granule neurons
作者:
Francisco Centeno,
Alfonso Mora,
José Fuentes,
Germà Soler,
Enrique Claro,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 18
页码: 4199-4203
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Apoptosis;Cerebellar granule neurons;Ceramide;C2-ceramide;Inositol;Lithium;NMDA receptors
数据来源: OVID
摘要:
PRIMARY cultures of cerebellar granule neurons, maintained in a serum-containing medium, underwent apoptosis when exposed to C2-ceramide, as assessed by mitochondrial reduction of MTT and intranucleosomal DNA fragmentation. After an 8h exposure to 50 μM C2-ceramide, cell viability decreased by 25–40%. Addition of lithium together with C2-ceramide resulted in a partial protection of apoptosis, which was maximal ab mM lithium (37% protection). When lithium was added h before the apoptotic stimulus the neuroprotective effect of the ion was clearly increased (66% protection). This effect was not due to intracellular inositol depletion or inhibition of NMDA receptors. Our data broaden the nature of apoptotic insults being reversed by lithium, stressing the neuroprotective effects of the ion.
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