Characterization of galactomannans by capillary electrophoresis
作者:
Cheryl L. Flurer,
期刊:
Food Additives & Contaminants
(Taylor Available online 2000)
卷期:
Volume 17,
issue 9
页码: 721-731
ISSN:0265-203X
年代: 2000
DOI:10.1080/026520300415255
出版商: Taylor & Francis Group
关键词: Capillary Electrophoresis Tara Guar Cassia Locust Bean Protein Profiles
数据来源: Taylor
摘要:
Capillary electrophoresis (CE) was utilized in the characterization of various galactomannans. Standards of gums were extracted with 50% CH3CN to remove the residual proteins from the gum matrix. Separation buffers were optimized with respect to pH, buffer concentration and presence of sodium dodecyl sulphate, yielding protein profiles from which the desired information could be obtained. Examples are given of the profiles generated by various gums and gum blends to aid in the verification of component presence, and to demonstrate levels of adulteration detectable under the buffer conditions used.
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