Continuous Growth and Differentiation of Trypanosoma (Megatrypanum) freitasi Rego, Magalhaes&Siqueira, 1957, In Vitro
作者:
NEIDE THOMAZ,
MARIA P. DEANE,
期刊:
The Journal of Protozoology
(WILEY Available online 1990)
卷期:
Volume 37,
issue 1
页码: 40-43
ISSN:0022-3921
年代: 1990
DOI:10.1111/j.1550-7408.1990.tb01111.x
出版商: Blackwell Publishing Ltd
关键词: Adhesion;bloodstream trypomastigotes;epimastigote colonies;opossum trypanosome
数据来源: WILEY
摘要:
ABSTRACTTrypanosoma (Megatrypanum) freitasi, a parasite of didelphid opossum, was known to be very difficult to cultivate in conventional media. Co‐cultivation with L929 cell line in Baltz's medium at 27.5° C resulted in luxuriant growth of the trypanosome with the production of epimastigote colonies that adhered to the surface of culture flasks or tubes, and transformation into metacylics. Further transformation was stimulated by raising the incubation temperature. At 37° C the population was of the bloodstream type and resistant to lysis by complem
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