Improved Fluorescence Assay of Liposome Lysis
作者:
RichardB. Thompson,
BruceP. Gaber,
期刊:
Analytical Letters
(Taylor Available online 1985)
卷期:
Volume 18,
issue 15
页码: 1847-1863
ISSN:0003-2719
年代: 1985
DOI:10.1080/00032718508062912
出版商: Taylor & Francis Group
关键词: Liposome Lysis;Fluorescence Assay;Complement-Mediated Immunolysis;4-Methylumbelliferyl Glucuronide
数据来源: Taylor
摘要:
A simple fluorescence assay for liposome stability is described. A fluorogenic enzyme substrate. 4-methylumbelliferyl-β-D-glucuronide, is encapsulated in liposomes. In the presence ofE. Coliβ-glucuronidase, any of the substrate that has been released into the medium is converted to 4-methylum-belliferone, which is monitored fluorimetrically. The method offers several advantages over current methods, including high gain, extremely low leakage, and facile quantitation. The use of the assay as part of a prototype immunoassay is decribed.
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