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Preservation of Brain Tumor Specimens for Drug Sensitivity Testing

 

作者: John Kapp,   Eddie Perkins,   Elton Tucker,  

 

期刊: Neurosurgery  (OVID Available online 1989)
卷期: Volume 24, issue 6  

页码: 810-813

 

ISSN:0148-396X

 

年代: 1989

 

出版商: OVID

 

关键词: In vitro drug sensitivity;Glioma;9L gliosarcoma;Cell culture;Chemotherapy

 

数据来源: OVID

 

摘要:

&NA;To examine the feasibility of shipment of brain tumor specimens to a central laboratory for drug sensitivity testing, an experimental gliosarcoma (9L) was grown subcutaneousley in rats, harvested, stored, and disaggregated. Growth of the disaggregated tumor cells in monolayer culture was evaluated after storage for various times at 2 to 6°C and 37°C in saline and minimum essential medium. Growth potential was maintained for 24 hours when tumor specimens were stored under refrigerated conditions and was best maintained when specimens were stored in saline. Specimens stored at 37°C grew best when stored in minimum essential medium, but growth potential was lost after 12 hours unless the specimens were refrigerated. Shipment of tumor specimens to a central laboratory for drug sensitivity testing appears to be feasible, since under most circumstances, specimens should reach the laboratory for processing within 24 hours of their removal. Storage and transport of specimens in saline on wet ice appears to be optimal. (Neurosurgery24:810‐813, 1989)

 

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