Spectrofluorimetric Determination of Nucleic Acids with Aluminum(III)/8-Hydroxyquinoline Complex
作者:
ChengZhi Huang,
YuanFang Li,
ShenYang Tong,
期刊:
Analytical Letters
(Taylor Available online 1997)
卷期:
Volume 30,
issue 7
页码: 1305-1319
ISSN:0003-2719
年代: 1997
DOI:10.1080/00032719708007218
出版商: Taylor & Francis Group
关键词: Nucleic acids;Aluminum(III);8-Hydroxyquinoline
数据来源: Taylor
摘要:
On the basis of the fluorescence enhancement effect of nucleic acids on the aluminum (III) / 8-hydroxyquinoline (8-HQ) complex, a spectrofluorimetric method for nucleic acids is proposed in the present paper. In the pH range 5.8–7.0, the fluorescence of the Al(III)/8-HQ complex, excited at 265 nm or at 365 nm, is enhanced by nucleic acids. The calibration curve was linear in the range 0.25 – 3.0 μg ml−1for calf thymus DNA, 0.25 – 3.5 μg ml−1for fish sperm DNA and 0.5 – 3.5 μg ml−1for yeast RNA, respectively. The limit of determination (3[sgrave]) was 0.024 μg ml−1for calf thymus DNA, 0.013 μg ml−1for fish sperm DNA and 0.130 μg ml−1for yeast RNA, respectively. Six synthetic samples were determined with satisfaction.
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