Umbilical Cord Transforming Growth Factor-β3: Isolation, Comparison with Recombinant TGF-β3 and Cellular Localization
作者:
StewartAlexander A.,
HaleyJohn D.,
Y.Guo,
StamKees,
FenyöDavid,
ChaitBrian T.,
MarshakDaniel R.,
KiuA. Yuk,
MarleyGarry,
IwataKenneth K.,
期刊:
Growth Factors
(Taylor Available online 1996)
卷期:
Volume 13,
issue 1-2
页码: 87-98
ISSN:0897-7194
年代: 1996
DOI:10.3109/08977199609034569
出版商: Taylor&Francis
关键词: transforming growth factor beta-3;TGF-β3;growth factor purification;umbilical cord;mass spectrometry;TGI
数据来源: Taylor
摘要:
AbstractThe transforming growth factor beta (TGF-β) family of growth modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-βisoforms (TGF-β1, -β2 and -β3) have overlapping yet distinct biological actions and target cell specificities, both in developing and adult tissues. The TGF-β3 isoform was purified to homogeneity from both natural and recombinant sources and characterized by laser desorption mass spectrometry, by protein sequencing, by amino acid analysis and by biological activity. TGF-β3 was the major TGF-βisoform in umbilical cord (230 ng/g), and was physically and biologically indistinguishable from recombinant TGF-β3 and from the tumor growth inhibitory (TGI) protein found in umbilical cord. Immunohistochemistry using antipeptide TGF-β3 specific antibody showed TGF-β3 localization in perivascular smooth muscle.
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