Total soluble proteins were extracted from the pollenof Ipomoea fistulosaand subjected to ammonium sulphate precipitation at 80% saturation. Total protein was separated on SDS Polyacrylamide gel. Each band was recovered from the gel by electroelution avoiding the time‐consuming and expensive biochemical methods of column chromatography. The allergenic proteins were identified by skin tests. The fourth and the fifth band in the protein profile ofI.fistulosa, IF4(47 kDa) and IF5(42 kDa) were the two major allergenic components ofI.fistulosa.The protein profiles showed slight variations in the pollen collected from different geographical regions.